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Fig. 3 | BMC Cancer

Fig. 3

From: PGE2 promotes breast cancer-associated lymphangiogenesis by activation of EP4 receptor on lymphatic endothelial cells

Fig. 3

Representative micrographs of HMVEC-dly tubular network formation in response to different agents. a Tube formation were measured on GFR-Matrigel at 24 h in the presence of SFM or RPMI medium (control), MDA-MB-231 CM, PGE2 (1μM), EP4 agonists PGE1OH (1μM), and L-902688. Bars equal 100 μm. b Quantification of the total tube length in the captured area at 10 different field of view, (FOV) for each experimental condition as determined by ImageJ. Each bar graph represents each experimental condition. Error bar represents mean ± SEM of three different experiments. (**) represents p < 0.001. Tubulogenesis quantitated by innumeration of branching points gave identical results (not shown)

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BMC Cancer

ISSN: 1471-2407

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