Fig. 10

Immuno-histological quantification of lymphatics and vascular endothelial cells in angioreactor contents based on double labeling. (Upper panel). Immunofluorescence localization of CD31 (green), Lyve1 (red), and Prox1 (red) in serial sections of angioreactors containing BME alone or BME + VEGF-C (5ng/200μl), BME + PGE1OH (10μM), BME + PGE2 (10μM), BME + L-902688(10μM). Images were captured using the confocal microscope. (lower panel). “Hot spot” scores for CD31-Lyve1 and CD31-Prox1 were calculated by means of Image J (40× magnification). Data are presented as mean of “hot spot” ± SEM. Scale bar equals 50 μm. (*) indicates significant difference p < 0.05 and (**) indicates p < 0.001 relative to BME (control) for each marker