Fig. 2

Microphotographs of the present case. a-b The lung adenocarcinoma (a, b: arrowheads): a haematoxylin and eosin staining (bar = 100 μm); b receptor for advanced glycation end-products (RAGE) staining (bar = 100 μm). Positive staining for RAGE is seen. c-e RAGE staining of other tissues (bar = 100 μm): c subcarinal lymph node; d duodenal mucosa; e bone marrow. None of these tissues show positive staining for RAGE. f-i Congo red staining (bar = 200 μm): f lung tissue surrounding the adenocarcinoma; g subcarinal lymph node; h duodenal mucosa; i bone marrow. Amorphous deposition was found in the tissues surrounding the tumour (f: arrowheads). j-m Amyloid P component staining (bar = 200 μm): j lung tissue surrounding the adenocarcinoma; k subcarinal lymph node; l duodenal mucosa; m bone marrow. All amorphous material shows positive staining for Congo red with apple-green birefringence in the polarized view, and amyloid P component