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Fig. 9 | BMC Cancer

Fig. 9

From: Protein kinase C inhibitor Gö6976 but not Gö6983 induces the reversion of E- to N-cadherin switch and metastatic phenotype in melanoma: identification of the role of protein kinase D1

Fig. 9

The E-cadherin induced by PKD1 depletion is cleaved in M2 metastatic melanoma cells. a. Protein extracts from control (C1 and C2 clones) and PKD1 depleted (P2, P3, P4, P5, P6 and P7 clones) M2 cells cultured in methylcellulose semi-solid medium for three weeks were analyzed by western blot using anti-PKD1, anti-E-cadherin and anti-actin antibodies. The results presented are those of typical experiments. b. Protein extracts from control (C1 and C2 clones) and PKD1 depleted (P2, P3, P4, P5, P6 and P7 clones) M2 cells cultured in adherent conditions were analyzed by western blot using anti-E-cadherin and anti-actin antibodies. T1 melanoma cells were used as positive control for E-cadherin expression. The results presented are those of typical experiments. c. Conditioned media from control (C1 and C2) and PKD1 depleted (P3, P4, P5 and P6) M2 cells were analyzed for E-cadherin by ELISA

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