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Fig. 3 | BMC Cancer

Fig. 3

From: Protein kinase C inhibitor Gö6976 but not Gö6983 induces the reversion of E- to N-cadherin switch and metastatic phenotype in melanoma: identification of the role of protein kinase D1

Fig. 3

Gö6976 but not Gö6983 induces rapid cell shape modification and E-cadherin-associated cell-cell interactions in M2 mesenchymal-like metastatic melanoma cells. M2 metastatic melanoma cells were seeded in 6-well plates at the density of 12,500 cells per well. After three to five days of culture, cells were treated with 1 μM Gö6976, 1 μM Gö6983 or vehicle (DMSO) for different periods of time (0, 1, 3, 24 or 48 h) (a) or for 24 h only (b and c). Cells were then fixed, permeabilized and stained for actin (green) alone (a), co-stained for actin and N-cadherin (red) (b) or co-stained for actin and E-cadherin (red) (c). Isotype-matched control antibodies were used. Cell nuclei were stained with DAPI (blue). Cells were analyzed under epifluorescence (a) or confocal (b and c) microscope. Images are representative fields from three or more independent experiments

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