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Fig. 11 | BMC Cancer

Fig. 11

From: Protein kinase C inhibitor Gö6976 but not Gö6983 induces the reversion of E- to N-cadherin switch and metastatic phenotype in melanoma: identification of the role of protein kinase D1

Fig. 11

PKD1 depletion inhibits anchorage-independent growth and migration of M2 metastatic melanoma cells. a. PKD1 depleted (P2, P3, P4, P5, P6 and P7 clones) and control (C1 and C2 clones) M2 cells were cultured in methylcellulose semi-solid medium for three weeks. Then, the colonies formed were counted. The quantifications presented are the means ± SD for three independent experiments. **, p < 0.01; ****, p < 0.0001 versus C1 and C2 control cells. b. Cells were incubated in RPMI medium in the upper well of a transwell chamber while in the lower well RPMI medium supplemented with 10% FBS was added. After 24 h, cells that invaded to the lower well were counted. The results presented are the means ± SD for three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus C1 and C2 control cells. c. Representative images of cell migration evaluated by wound healing assay. Images of wound repair were taken at 0 and 24 h after wound and the cells that migrated to the wounded area were counted. The results presented are the means ± SD for three independent experiments. *, p < 0.05; **, p < 0.01 versus C1 and C2 control cells

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