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Fig. 1 | BMC Cancer

Fig. 1

From: Protein kinase C inhibitor Gö6976 but not Gö6983 induces the reversion of E- to N-cadherin switch and metastatic phenotype in melanoma: identification of the role of protein kinase D1

Fig. 1

Comparative analysis of E- and N-cadherin expression and oncogenic properties between primary tumor and respective metastatic melanoma cell lines a. Protein extracts from the primary tumor (I5 and T1) and respective lymph-node metastatic (M2 and G1) melanoma cell lines were analyzed by western blot using anti-E-cadherin, anti-N-cadherin and anti-actin antibodies. The results presented are those of typical experiments. b. The proliferation of I5, M2, T1 and G1 cells was analyzed over six days by MTT assay. Results presented are the means ± SD for three independent experiments. c. I5, M2, T1 and G1 cells were cultured in methylcellulose semi-solid medium for three weeks. Then, the colonies formed were counted. The results are presented as the percentage of seeded cells that form colonies (clonogenicity) and are the means ± SD for three independent experiments. ***, p < 0.005 versus respective primary tumor cells. d. Representative images of cell migration evaluated by wound healing assay. Images of wound repair were taken at 0 and 24 h after wound

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