Fig. 4

Inhibition of tumor growth in vivo upon SATB1 knockdown. a Subcutaneous U-87 MG tumor xenografts were established in athymic nude mice. Upon randomization, mice were treated by i.t. injection of 2 μg siRNAs specific for SATB1 (si467) vs. negative control siRNAs (siCtrl). For siRNA delivery, siRNAs were formulated in polymeric nanoparticles based on a low-molecular weight polyethylenimine (PEI F25-LMW). Untreated mice (‘wt’) served as additional negative control for the absence of non-specific treatment effects. Right: representative pictures of mice (n = at least 13 tumor xenografts per group). b The determination of masses of the tumor xenografts explanted upon termination of the experiment confirmed the tumor growth inhibition