Fig. 3

Analysis of molecular consequences of SATB1 knockdown in G55T2 cells. The siRNA-mediated knockdown of SATB1 affects the expression levels of a broad spectrum of (proto-) oncogenes and various molecules involved in cell cycle, EMT, signal transduction and cell adhesion, as determined on the mRNA level by quantitative RT-PCR at 48 – 72 h after transfection (a) and on the protein level by Western blotting (b). For details, see text. In (a), differences that reached significance are indicated (n = 5–6 experiments determined at 72 h after transfection; Slug and Twist: n = 9 experiments determined at 48 – 72 h after transfection)