Fig. 2

Effects of SATB1 knockdown on cell cycle and apoptosis. a Transient SATB1 knockdown leads to a decreased percentage of cells in G2/M and an increase of cells in G₀/G₁, as determined in U-87 MG (upper panel) and G55T2 glioblastoma cells (lower panel). Cell cycle inhibition is more pronounced when using the more efficient si467, indicating a SATB1 gene-dose effect. Measurements were performed 8 h after addition of nocodazole (see text for details; n = 2 experiments per cell line). Lower right: representative flow cytometry histograms. b Induction of apoptosis upon transient SATB1 knockdown, as determined by increased caspase-3/-7 activity. Effects are seen in both U-87 MG (left) and G55T2 glioblastoma cells (right) and are dependent on the degree of SATB1 reduction