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Fig. 1 | BMC Cancer

Fig. 1

From: Analysis of cellular and molecular antitumor effects upon inhibition of SATB1 in glioblastoma cells

Fig. 1

siRNA-mediated SATB1 knockdown exerts tumor cell inhibitory effects. SATB1 knockdown upon transfection of SATB1-specific siRNAs si467 or si989 in U-87 MG and G55T2 glioblastoma cells, as determined on (a) mRNA (n = 3–4 experiments, performed in duplicates and analyzed 72 h after transfection) and (b) protein level (n = 4 experiments, analyzed 96 h after transfection, one representative shown). Actin was used as loading control, and transfections with siRNAs targeting the irrelevant protein luciferase served as negative controls (siCtrl). Two specific siRNAs were explored, with si467 being more efficient than si989. c Marked inhibition of anchorage-dependent proliferation, particularly when using the more potent si467. d Decreased colony formation ability of U-87 MG cells in soft-agar assay, indicative of impaired anchorage-independent growth (right panel: representative photos of soft-agar colonies)

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