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Fig. 4 | BMC Cancer

Fig. 4

From: Novel derivative of aminobenzenesulfonamide (3c) induces apoptosis in colorectal cancer cells through ROS generation and inhibits cell migration

Fig. 4

3c activates cytochrome c release, PARP and caspase cascade. a. HT-29 cytosolic extracts were immunoblotted with the indicated antibodies. b. SW620 cytosolic extracts were immunoblotted with the indicated antibodies. c. HT-29 cells were treated with 3c, mitochondrial and cytosolic extracts were isolated and immunoblotted with the indicated antibodies. d. Cells were treated with 3c for 24 h, cytosolic extracts were isolated and ELISA was performed for caspases activity. e. HT-29 cells were pre-treated with NAC (5 mM) for 1 h followed by 3c treatment. Cytosolic extracts were immunoblotted with the indicated antibodies. f. SW620 cells were pre-treated with NAC (5 mM) for 1 h followed by 3c treatment. Cytosolic extracts were immunoblotted with the indicated antibodies

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