Fig. 1
NAC reverses the cytotoxicity induced by 3c. a. HT-29 cells were treated with 3c (5 μM) in the absence and presence of NAC (5 mM), Cell viability was measured by MTT assay. The results are expressed as mean of 3 independent determinations (mean ± SD). b. 5 x 103 cells were seeded in E-plate 16, after overnight incubation, cells were pre-treated with NAC followed by treatment with 3c. Real-time proliferation monitoring of HT-29 cells was performed by measuring cell index to evaluate the cytotoxic effect of 3c using xCELLigence RTCA-DP system. c-d. HT-29 cells were treated with various concentration of 3c for 24 h. 0.5% DMSO was used as a control. Cells were processed for flow cytometry using Annexin V/PI staining. The percentage of Annexin V+ population indicates apoptosis induction. Results shown are representative of 3 independent experiments. In some cases where indicated cells were pre-treated with NAC for 1 h followed by 3c treatment