Fig. 4

miR-663a can target the CXCR4-Akt pathway. a Protein levels of CXCR4, Akt, phospho-Akt, and αtubulin in the total cell lysates were determined by western blotting analysis. Representative data is shown in triplicate experiments. Ratio of phospho-Akt/Akt was calculated after digitalization by using JustTLC and shown as mean ± SD of triplicate experiments (** p < 0.001). b Putative model of anti-cancer effect of AMPs in HCT116 cells. miR-663a upregulated by AMPs suppresses CXCR4 expression leading to reduced phosphorylation of Akt. Consequently, p21 and the inactive form of cdc2 accumulation are caused, which results in the cell cycle arrest at the G2/M phase