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Fig. 2 | BMC Cancer

Fig. 2

From: miR-663a regulates growth of colon cancer cells, after administration of antimicrobial peptides, by targeting CXCR4-p21 pathway

Fig. 2

miR-663a induced cell cycle arrest following p21 expression and accumulation of the inactive form of cdc2 in HCT116 cells. a Cell cycle was examined by the MUSE cell analyzer and representative data are shown. The percentage of cells in G0/G1, S, and G2/M phases are presented as mean ± SD of triplicate experiments. (** p < 0.001). b The mRNA expression of p21 and p53 were determined by qPCR and relative expression levels are shown as mean ± SD of triplicate experiments. (** p < 0.001). c Protein levels of p53, p21, cdc2, phospho-cdc2 (Tyr15), and αtubulin in the total cell lysates were determined by western blotting analysis. Representative data of three experiments are shown. Ratio of phospho-cdc2 (Tyr15)/cdc2 was calculated after digitalization by using JustTLC and shown as mean ± SD of triplicate experiments. (** p < 0.001)

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