Fig. 1

miR-663a is the major upregulated miRNA in HCT116 cells treated with LL-37 and FF/CAP18. a Viability of HCT116 cells after treatment with LL-37, FF/CAP18, or Sc/FF for 48 h at the concentration of 1.56–100 μg/mL. Each data is presented as mean ± SD of triplicate experiments. (* p < 0.05; ** p < 0.001). b Relative expression of miR-663a in HCT116 cells treated with LL-37 (40, 80 μg/mL) and FF/CAP18 (40 μg/mL). Each data is shown as mean ± SD of triplicate experiments. (* p < 0.05; ** p < 0.001). c Morphology of established over-expressing miR-663a HCT116 cells using the lentivirus vector system. HCT116 cells transduced with lentiviruses harboring control vector (miR-ctrl) and miR-663a-expressing vector (miR-663a) expressed red fluorescence (rPuro). d The RT-qPCR analysis showed that HCT116 cells transduced with miR-663a-expressing vector expressed miR-663a. Each data is shown as mean ± SD of triplicate experiments. (* p < 0.05). e Proliferation of HCT116 cells (wt) and HCT116 cells transduced with lentiviruses harboring control vector (miR-ctrl) and miR-663a-expressing vector (miR-663a) for 4 days. Each data is presented as mean ± SD of triplicate experiments. (** p < 0.001)