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Table 3 Antibodies used in the study and their specifications

From: Chemotherapy-induced Sinusoidal Injury (CSI) score: a novel histologic assessment of chemotherapy-related hepatic sinusoidal injury in patients with colorectal liver metastasis

Antibody

Cat number/clone

Source

Dilution

Antigen retrieval

CD34

M7165/clone QBEnd-10

DAKO (Carpinteria, CA, USA)

1:25

Steam slides in preheated Target Retrieval Solution, pH 9 (Carpinteria, CA, USA) for 30 min

SMA

M0851/clone 1A4

DAKO (Carpinteria, CA, USA)

1:50

None

GS

MAB302/clone GS-6

EMD Millipore (Billerica, MA, USA)

1:2000

Steam slides in pre-heated Target Retrieval Solution, Citrate pH 6 (Carpinteria, CA, USA) for 60 min