Table 2 Secondary antibodies used for IHC
Description | Flourophore | Isotype specificity | Dilution | Source | Detection of |
|---|---|---|---|---|---|
Goat anti-mouse | A488 | IgG3 | 1:500 | Invitrogen, A21151 | CK14 |
Goat anti-mouse | A350 | IgG2a | 1:250 | Invitrogen, A21130 | CK19 |
Goat anti-mouse | A488 | IgG2a | 1:500 | Invitrogen, A21131 | CK19 |
Mouse anti-human c-Myc | Cy3 | IgG1 | 1:250 | Sigma-Aldrich, C6594 | dAb |
Goat anti-rabbit | A488 | IgG (H + L) | 1:400 | Invitrogen, A11008 | dAb-rFc |