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Fig. 5 | BMC Cancer

Fig. 5

From: Functional significance of CD105-positive cells in papillary renal cell carcinoma

Fig. 5

Colony- and sphere-forming abilities of pRCC cell lines. a Representative images of colonies formed in semi-soft agar at different time points of culture. From four tested cell lines, only two (ACHN and HKCSC) were able to generate colonies in semi-soft agar. The differences in colony morphologies were noticed: ACHN created round-shaped colonies and HKCSC irregular-shaped. A quantitative analysis of viable colonies was done using the alamarBlue® assay: HKCSCs created significantly more colonies than ACHN. b Representative images of cells grown in sphere-promoting conditions on the fifth day of culture. ACHN were able to create aggregate-like structures. Caki-2 spheres were smaller and more round-shaped. The other cell lines, HKCSC and ASE, did not form spheres in these conditions. A quantitative analysis of Caki-2 and ACHN spheres number showed no significant differences, although the ACHN spheres were significantly bigger than the Caki-2 spheres

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