Fig. 2

CD105 expression on protein and mRNA level. For further analysis, Caki-2 (high expression), ACHN (low expression), HKCSC (control), and ASE (control) were used. a Representative dot plots of CD105 and CD133 expression in tested cell lines. b Percentage of CD105+ cells in tested cell lines measured by flow cytometry. Within control cell lines, only normal renal cells of embryonic origin (ASE) had a CD105+ population, while in the commercially available renal cancer stem cell line (HKCSC), this population was hardly detected. c Relative expression of CD105 gene was measured by real-time PCR in relation to the PPIA housekeeping gene. CD105 expression was significantly upregulated in Caki-2 and downregulated in ACHN; a similar observation was made in the FACS analysis. d ICC staining was done to confirm Caki-2 and ACHN flow cytometry results. Around one-third of Caki-2 cells were positive for the CD105 marker with significant expression. However, in ACHN CD105+ cells were not detected with this method