Fig. 7

The interaction of neutrophils with opsonized melanoma cells. Oxidative burst. B16-F10 melanoma cells were incubated (30 min, 37 °C) with 0.02 mM mannan-BAM in culture medium and subsequently washed. The suspension of bone marrow neutrophils (90% purity), primed with GM-CSF + TNF-alpha + laminarin in culture medium was added to B16-F10 (both free and mannan-BAM covered) in the ratio 5:1. Where indicated, 0.1 mM epicatechin was added. All mixtures were incubated for 2 hours at 37 °C. After the incubation, living, trypan blue excluding melanoma cells (a) and neutrophils (b) were counted with a haemocytometer. *P ≤ 0.05 compared to B16-F10 + epicatechin. oP ≤ 0.005 compared to B1-F10. ■P ≤ 0.0005 compared to B16-F10 + epicatechin. ¤P ≤ 0.005 compared to mannan-BAM covered B16-F10. xP ≤ 0.05 compared to B16-F10 + neutrophils