Fig. 2

Effect of ERβ knockdown on invasion of MDA-MB-231 cells. a Effect of treatment with ESR2 siRNA for 72 h on ERβ protein expression in MDA-MB-231 cells as assessed by Western blot analysis. b Effect of ERβ knockdown on cellular invasion of MDA-MB-231 cells through a basement membrane in vitro. The day after transfection, cells were seeded on top of a 3 mm reconstituted basement membrane gel, and invasion was determined after further 48 h as described in the Materials and Methods section. Values are expressed in percentage of invasion of control-transfected cells. *vs. negative control siRNAs (n = 3) (unpaired t‑test, two‑tailed)