Fig. 1

Effect of different ERβ agonists on invasion of triple negative MDA-MB-231 and HS578T breast cancer cells. Cellular invasion through a 3 mm gel of reconstituted basement membrane was determined using a modified Boyden chamber. Cells were pre-treated for 48 h with the indicated concentrations of ERβ agonists, seeded on top of the basement membrane gel in the presence of the same agonist concentrations, and invasion was determined after further 48 h as described in the Materials and Methods section. Values are expressed in percent of invasion of vehicle-treated cells. *p < 0.05 vs vehicle control. (n = 4). (Kruskal-Wallis H-test with Bonferroni post-hoc test)