Fig. 3

Increased proliferation rate and self-renewal ability of HT29 cells over SW480 cells. a Growth of colon cancer cell lines under normal culture conditions. An equal number of cells per well were plated and counted at days 0, 1, 3 and 5 with a hemocytometer. Dead cells were excluded by trypan blue analysis. Data represent mean S.E.M. (N = 3). b These images represent a sphere formed from a single cell, after two weeks in culture. Both images were taken under bright field with a 10x objective lens, on the Zeiss Epi-Fluorescence microscope. Quantification of spheres was done by measuring the diameter of the spheres from random fields. Data represents mean ± S.E.M. (N = 3)