Table 1 Overview of the HC2, cobas and Aptima test specifications and accompanying automated testing solutions
Assay | Nr. of devices | HrHPV types detected | Target gene | Detection technology | Input volume | Dead volume | Control |
|---|---|---|---|---|---|---|---|
HC2 | 4 | 16/18/31/33/35/39/45/51/52/56/58/59/68 | Entire hrHPV genome (DNA) | Nucleic acid hybridization assay with signal amplification | 3.0 mL | 5.0 mL | Process control for each sample batch |
cobas | 3 | 16/18/31/33/35/39/45/51/52/56/58/59/66/68 | L1 (DNA) | Real time Polymerase Chain Reaction | 0.25 mL | 0.5 mL | ß-globin for process validity of individual sample and input sufficiency |
Aptima | 2 | 16/18/31/33/35/39/45/51/52/56/58/59/66/68 | E6/E7 (RNA) | Transcription Mediated Amplification | 0.4 mL | 5.0 mL | Spiked internal control for process validity of individual sample |