Fig. 4

MMTV-PyMT;Apc ^Min/+ cells have higher aldehyde dehydrogenase (ALDH) enzyme activity than MMTV-PyMT;Apc ^+/+ cells. ALDH activity was measured using an Aldefluor™ Kit. For each cell line a Control (+DEAB) and test (−DEAB) sample were run. a Representative FACS analysis of ALDH activity in MMTVPyMT; Apc ^+/+ and MMTV-PyMT;Apc ^Min/+ cells using the Aldefluor™ assay. ALDH activity is increased in MMTV-PyMT;Apc ^Min/+ cells. b The population of cells that shifted outside of the control population was calculated for each test sample, indicating ALDH activity. MMTV-PyMT;Apc ^Min/+cells show a larger percentage of cells shifted outside of the control range than MMTV-PyMT;Apc ^+/+cells. Data are shown as the means ± SEM from 3 independent experiments; *P < 0.05