Clinicopathological and prognostic significance of mTOR and phosphorylated mTOR expression in patients with esophageal squamous cell carcinoma: a systematic review and meta-analysis

Table 2 Baseline characteristics of included studies

Authors (Year)	Materials	Detection	Antibody	Dilution	Positive site	Cut-off value	Estimates	Extractions	Analysis	Endpoints	Follow-up
Boone et al. (2008) [38]	Paraffin-embedded tissue	IHC	Ser2448	1:50	Cytoplasm	20 % staining	OR	DDE	U	––––	––––
Chen et al. (2010) [39]	Paraffin-embedded tissue	IHC	Rabbit anti-mTOR	1:100	Cytoplasm	10 % staining	OR	DDE	U	––––	––––
Chuang et al. (2015) [40]	Paraffin-embedded tissue	IHC	Rabbit anti-mTOR	1:100	NI	Median H-score	OR, HR	DDE	U	OS	120 months
Hirashima et al. (2010) [41]	Paraffin-embedded tissue	IHC	Ser2448	1:50	Cytoplasm	10 % staining	OR, HR	Reported, DDE	U & M	OS, CSS	133 months
Hou et al. (2014) [42]	Paraffin-embedded tissue	IHC	Rabbit anti-mTOR	1:200	Cytoplasm	10 % staining	OR	DDE	U	––––	––––
Kim et al. (2013) [43]	Paraffin-embedded tissue	IHC	Ser2448	1:100	NI	5 % staining	OR, HR	Reported, DDE	U & M	OS, CSS	120 months
Li et al. (2012) [44]	Paraffin-embedded tissue	IHC	Ser2448	1:50	Cytoplasm	10 % staining	HR	Reported	M	OS, DFS	120 months
Li et al. (2015) [45]	Paraffin-embedded tissue	IHC	Ser2448	1:50	Cytoplasm	10 % staining	OR, HR	DDE	U	OS, DFS	146 months
Lu et al. (2015) [46]	Paraffin-embedded tissue	IHC	Rabbit anti-mTOR	1:100	Cytoplasm	25 % staining	OR, HR	Reported, DDE	U & M	OS, DFS	36 months

CP clinicopathological, CSS cancer-specific survival, DDE demographic data extrapolated, DFS disease-free survival, HR hazard ratio, IHC immunohistochemistry, M multivariate, NE negative expression, NI no information, NIT neo-adjuvant induction therapy, NOS Newcastle-Ottawa Scale, OR odds ratio, OS overall survival, PE positive expression, ROS retrospective observational study, U univariate

ISSN: 1471-2407