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Fig. 3 | BMC Cancer

Fig. 3

From: Leptin and insulin up-regulate miR-4443 to suppress NCOA1 and TRAF4, and decrease the invasiveness of human colon cancer cells

Fig. 3

Leptin and insulin exposure and overexpression of miR-4443 down-regulate the miR-4443 targets, NCOA1 and TRAF4, in HCT-116 cells. a RT-qPCR for human NCOA1 and TRAF4 in HCT-116 cells cultured for 48 h with or without 100 ng/ml leptin or 20 ng/ml insulin. *:p < 0.05; t-test; bars: SD from triplicates. b Immunoblot for human NCOA1 and TRAF4 in HCT-116 cells as in (a). β-actin was used as loading control. c RT-qPCR for human NCOA1 and TRAF4 in HCT-116 cells transfected with miR-4443 mimicking oligo, or mock-transfected cells. *:p < 0.05; t-test; bars: SD, n = 5. d Immunoblot for human NCOA1 and TRAF4 in HCT-116 cells transfected with miR-4443 mimicking oligo, or mock-transfected cells. β-actin was used as loading control. e Secrete-Pair Luciferase/SEAP ratios in media of HCT-116 cells 48 h post co-transfection with constructs containing the 3′ UTR of TRAF4, NCOA1, or no UTR, with miR-4443 - mimicking or control oligo (miR-Neg). *:p < 0.05; **:P < 0.01; t-test; bars: SE, n = 6

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