Fig. 1

Leptin has effects similar to those of insulin on miRNA expression profiles in CRC-derived cell lines, and these effects are LEPR-dependent. a Scatter plot comparing the effects of leptin and insulin (both at 200 ng/ml), on the expression (relative to non-treated controls) of individual miRNAs as quantified by Nanostring profiling, in HCT-116 cells. b Scatter plot as in (a), in HT-29 cells. c Scatter plot as in (a), in DLD-1 cells. d Scatter plot comparing the effects of 200 ng/ml insulin on the expression (relative to non-treated controls) of individual miRNAs as quantified by Nanostring profiling, in HT-29 and HCT-116 cells. Circle representing miR-4443 marked in black. e Scatter plot as in (d), in HCT-116 and DLD-1 cells. Circle representing miR-4443 marked in black. f Scatter plot comparing the effects of 200 ng/ml leptin on the expression (relative to non-treated controls) of individual miRNAs as quantified by Nanostring profiling, in HT-29 and HCT-116 cells. Circle representing miR-4443 marked in black. g Scatter plot as in (f), in HT-116 and DLD-1 cells. Circle representing miR-4443 marked in black. h Electrophoresis of PCR products obtained with LEPR primers targeting a conserved region (common to all known variants), as well as specific to the longer and active variant of LEPR, and cDNA from DLD-1, HCT-116, and HT-29 cells. i Immunoblot for human LEPR in HCT-116, HT-29 and DLD-1 cells. β-actin was used as loading control