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Table 1 Conventional and modified BRASIL enrichment results based on binding affinity to the breast cancer MDA-MB-231 cell line and a counter selection with the non-tumorigenic MCF-10-2A cell line with the 7-mer and 12-mer libraries

From: Screening and characterization of novel specific peptides targeting MDA-MB-231 claudin-low breast carcinoma by computer-aided phage display methodologies

Method 7-mer library 12-mer library
Phage clone Sequence Phage clone Sequence
Conventional 3.1 (5/27) PRLNTSP 6.2 (1/17) TTFNSFGRVAIE
3.1 (6/27) PTLDVAP 6.2 (2/17) TTFCSFGRVRIE
3.1 (8/27) PQLNVSP 6.2 (3/17) TTFNSFGRVRIE
3.1 (9/27) PGAQVSP 6.2 (4/17) TTFNSFGRVHWE
3.1 (10/27) PRTNVAP 6.2 (5/17) TTFNSFGKVRIE
3.1 (11/27) PRKTVSP 6.2 (7/17) TTYNSFGRVRIE
3.1 (12/27) PAMNVSP 6.2 (8/17) TTEYSFGRTSTL
3.1 (13/27) PQENASP 6.2 (9/17) DTFNSFGRVRIE
3.1 (19/27) PSLNVSP 6.2 (10/17) TTFNSFGRVRIQ
3.1 (20/27) ARLNVAP 6.2 (12/17) TTFNSFGRVRIE
3.1 (21/27) TMLMVRP 6.2 (13/17) TNFNDFKRVRGE
3.1 (22/27) ARLNTQP 6.2 (14/17) TTFSSFGRVRIG
3.1 (23/27) PMMAVAP 6.2 (15/17) TTFNSNGRVWIE
3.1 (26/27) PRLNVSP 6.2 (16/17) TTFNSFGRVRGG
3.1 (27/27) PRLNVSP 6.2 (17/17) TTFNSFYRVRIE
Consensus (overall %) PRLNVSP (70%) Consensus (overall %) TTFNSFGRVRIE (86%)
BRASIL 1.3 (2/52) PRQNQSP 5.3 (1/45) WWFNSFGRVRIE
1.3 (3/52) QLKNVTP 5.3 (3/45) WAFNSFGRVRIE
1.3 (5/52) PRLNVAT 5.3 (5/45) WWFNKFGKVRIE
1.3 (6/52) PRLNVTT 5.3 (14/45) WWFNSFGRVRIE
1.3 (7/52) PRWAVSP 5.3 (16/45) IWFNSFFRVRIE
1.3 (8/52) PRLNHSP 5.3 (19/45) WWFFSFGRVRIE
1.3 (10/52) PQMTAMP 5.3 (20/45) GWFNSFGRWSWL
1.3 (11/52) MFLNGAP 5.3 (23/45) WWFNSFGRVRIE
1.3 (15/52) TRLQVSP 5.3 (43/45) WWFFSFGRWSWL
1.3 (19/52) WRMAHSP 5.3 (45/45) WWFNSFGRVRIE
  Consensus (overall %) PRLNVSP (60%) Consensus (overall %) WWFNSFGRVRIE (87%)
  1. Bold words represent the consensus (overall %) for the two methods