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Fig. 5 | BMC Cancer

Fig. 5

From: Global DNA methylation profiling uncovers distinct methylation patterns of protocadherin alpha4 in metastatic and non-metastatic rhabdomyosarcoma

Fig. 5

PCDHA4 involvement in tumor cell growth and invasion. RH36 cells that express high levels of PCDHA4 were transiently transfected with siRNA for PCDHA4 (siPCDHA4) or negative siRNA control (siCONTROL). The efficiency of silencing was evaluated using qRT-PCR (a). Transfected cells were used to analyze cell cycle distribution (b) or invasion c). The results are shown as mean ± IC of the percentage (%) of proliferation, measured using flow cytometry 48 h after transfection (b) and as the area covered by Matrigel-invading cells (c). Cell proliferation and invasion of PCDHA4 silenced cells are shown relative to control values (siCONTROL). Two independent experiments were performed in triplicate and mean results are shown. *P < 0.05; (The error bar represent a 95 % confidence interval, IC)

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