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Fig. 2 | BMC Cancer

Fig. 2

From: Global DNA methylation profiling uncovers distinct methylation patterns of protocadherin alpha4 in metastatic and non-metastatic rhabdomyosarcoma

Fig. 2

PCDHs genes expression level by qRT-PCR analysis. Relative expression levels of 15 PCDHs genes in PAX3/FOXO1 ARMS cell lines compared to ERMS cell lines (a) and in metastatic RMS tumor samples compared to non-metastatic RMS tumor samples (n = 15) (b). Data distribution is represented by box plot analysis performed using REST software. The relative expression of the PCDHA4 gene was evaluated in a larger cohort of RMS samples (n = 61). c PCDHA4 mRNA levels were lower in metastatic RMS samples (stage IV) with respect to non-metastatic RMS (stage I-II-III). d Comparison of metastatic ARMS vs non-metastatic ARMS confirmed the association of low PCDHA4 levels with the metastatic phenotype. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a housekeeping gene for data normalization. Relative expression (RQ) was calculated using ∆∆Ct method. Statistical analysis (Mann–Whitney U-test) was performed using Prism 6 software. * P < 0.05; **P < 0.01; ***P < 0.001

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