Fig. 5

Visualization of submillimeter sized intra-peritoneal metastases using EpCAM specific 323/A3-800CW in combination with the Artemis system: a MCF-7-luc2-cGFP cells were injected intraperitoneal in mice. After the development of multiple tumors, 1 nmol (150ug) 323/A3-800CW was injected intravenously. NIR fluorescence imaging was performed after 72 h of incubation using the Pearl and Artemis system. Confirmation of tumor tissue was performed by Green Fluorescent Protein (GFP) fluorescence imaging or Bioluminescence Imaging (BLI) using the IVIS spectrum. Micrometastases (arrow) of the colon could be discovered by NIR fluorescence imaging after resection of the peritoneum. b ROC curve analysis of TBR of fluorescent spots in the peritoneum. True positive rate was plotted versus false-positive rate using ascending positive cutoff values. B = bladder. Scale bars are 5 mm. Artemis fluorescence intensities were intra-operatively optimized for optimal contrast. Intensities can only be compared within images