Fig. 4

Intra-operative NIR fluorescence delineation of colon, breast and Head & Neck cancer: Colon a, b breast c and head & neck tumors d could clearly be visualized during operation using EpCAM specific 323/A3-800CW (TBR colon 13.5, breast 6.7 and Head-and-neck 4.9) and the Artemis imaging system. 323/A3-800CW (A, C, D, 1 nmol) and the non-specific antibody MOPC21-800CW (B, 1 nmol) were intravenously injected. After an incubation of 72 h NIR fluorescence imaging was performed. Depicted images are: bright light, fluorescence, overlay (fluorescence and bright light), ex vivo fluorescence and ex vivo overlay. Scale bars are 5 mm. Artemis fluorescence intensities were intra-operatively optimized for optimal contrast. Intensities can only be compared within images