Fig. 6

Decreased GLUT1 external localization by Prima-1 and 3-BrPA exposed for 3 h to hypoxia in A549 cells is not reverted by 2 mM NAC. Inmmune fluorescence was used to analyze preferential cell surface (red arrows) or perinuclear localization (white arrows) in p-formaldehyde fixed and permeabilized cells, treated as indicated in each case under hypoxia