Fig. 3

a NAC counteracts toxicity of Prima-1 and 3-BrPA in hypoxic (G12S)-mutant KRAS-A549 cells. Crystal violet staining of surviving cells was used to compare the response to a 72 h treatment with Prima-1 or 3-BrPA in A549 cultures under hypoxia in complete Dulbecco’s medium containing 20 mM glucose, 4 mM glutamine supplemented with 10% serum. b and c Prima-1 and 3-BrPA cooperate to inhibit cell cycle progression and promote hypoxic cell death is antagonized by NAC. Cell cycle analysis and assay of below_2n dead cells was performed as indicated under Methods for cells cultured under hypoxia for 48 h in 20 mM glucose, 4 mM glutamine supplemented with 10% serum, or 5 mM glucose, 2 mM glutamine and 5% dialyzed serum. *denotes significance between treated cells relative to controls