Fig. 4

Effect of tanshinone IIA and ADM alone and in combination on the cell cycle arrest and apoptosis induction in A549 cells. The cell cycle distributions after 48 h treatment with 36 μM of tanshinone IIA and 1.5 μM of ADM alone or in combination (a). The apoptosis rate after 48 h treatment with 36 μM of tanshinone IIA and 1.5 μM of ADM alone or in combination (b). Representative photographs of TUNEL staining cells in various groups (c). Histogram of quantification of TUNEL-positive cells was shown with the percentage of TUNEL-positive nuclei (green) relative to DAPI-positive total nuclei (blue) (d). All data represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, or ***P < 0.001 versus the vehicle control