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Fig. 6 | BMC Cancer

Fig. 6

From: Esophageal squamous cell carcinoma invasion is inhibited by Activin A in ACVRIB-positive cells

Fig. 6

Fibro-ActA conditioned media from three-dimensional organotypic cultures inhibits in vitro angiogenesis. a Gelatin zymography of conditioned media collected from 3D-organotypic cultures at day 7 (fibroblasts alone), day 9 (media collection following the addition of epithelial cells), and day 17 (final collection). Arrows indicate the locations of pro- and cleaved MMP-2 and MMP-9. b Representative images of endothelial tube formation assays using HMEC-1 cells. HMEC-1 endothelial cells cultured with day 9 conditioned media from ECdnT/Fibro-ActA and TE-2/Fibro-ActA cultures had less overall tube formation, compared to parent and empty vector controls. This effect was not observed upon treatment with TE-11/Fibro-ActA conditioned media. Quantification of endothelial extremities, nodes, junctions, and branches for the assays with ECdnT (c-f), TE-2 (g-j), and TE-11 (k-n) conditioned media shown in (b)

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