Fig. 4

Alteration of subpopulation of CD24⁺CD44⁺ cells by GSI IX treatment in the CC cell lines. a Representative data in RBE cells treated with DMSO or GSI IX. b Percentage of CD44⁺CD24⁺ subpopulation in the CC cell lines exposed to GSI IX. The subpopulation of CD24⁺CD44⁺ cells after treatment with 20 and 40 μM of GSI IX were significantly decreased to 7.0 and 5.0 %, respectively, in RBE cell lines, compared to control (21.5 %) (P < 0.05). In the other CC cell lines, GSI treatment also decreased the subpopulation of CD24⁺CD44⁺ cells. c Representative data in RBE cells treated with DMSO, GSI IX, GEM or combination of GSI IX and GEM. d Percentage of CD44⁺CD24⁺ subpopulation in the CC cell lines treated with DMSO, GSI IX, GEM, or combination of GSI IX and GEM. The subpopulation of CD24⁺CD44⁺ cells increased to 28.3 % after monotherapy with GEM. The combination with GSI IX and GEM significantly diminished the subpopulation to 22.1 % (P < 0.05). The results of GEM monotherapy or combination of GSI IX and GEM were consistent in the other CC cell lines