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Fig. 4 | BMC Cancer

Fig. 4

From: miR-199a-5p confers tumor-suppressive role in triple-negative breast cancer

Fig. 4

miR-199a-5p inhibited stem-cell like properties in breast cancer cells (a) Ectopic expression of miR-199a-5p reduced the single cell colony formation ability in MDA-MB-231 breast cancer cells (p = 0.0182). Single-cell clonogenic assay was used to determine single cell colony formation ability, cell clones reached more than 50 cells were considered as cell colonies. Representative micrographs (left panel) of colonies in single-cell clonogenic assay. b ALDH activity assay showed a significant reduced ALDH-positive cell population in miR-199a-5p mimic group than in the control group (17.2% vs 10.7%, p = 0.039). c ALDH1A3 expression was significantly suppressed after miR-199a-mimic treatment (p = 0.0088). d CD24-/CD44+ cell population showed significant decrease in miR-199a-5p overexpressing cells compared to its control group (p = 0.0015). e miR-199a-5p inhibited in vivo tumor growth. Left panel: three represented nude mice showed the reduction of tumor growth by overexpression of miR-199a-5p (control: left side, miR-199a-5p: right side). Right panel demonstrated the increase in volume of xenograft in the miR-199a-5p group. f Immunohistochemistry staining of mice xenograft from fat pad injection with MDA-MB-231 miR-199a-5p stable cells. (*P ≤ 0.05, **P ≤ 0.01). All experiments were repeated in triplicates

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