Fig. 4
From: Secreted Frizzled-Related Protein 2 (sFRP2) promotes osteosarcoma invasion and metastatic potential
Knockdown of sFRP2 in metastatic mouse cells decreases in vitro migratory and invasive potential for OS. a CCK-8 cell proliferation assay of multiple shsFRP2/RF1044 and RF1044 vector control cells. Western blot analysis showing expression of sFRP2 in control and knockdown cells. b Cell migration assay of shsFRP2/RF1044 and control RF1044 cells. c Transwell invasion assay of control RF1044 (left panel) and shsFRP2/RF1044 (right panel) cells. Crystal violet staining for control and shsFRP2 expressing cells is shown. Quantification of tumor cell invasion is shown to the right. d 3D matrigel assay for vector control (top panel) and shsFRP2/RF1044 cells (bottom panel)