Fig. 3
From: Secreted Frizzled-Related Protein 2 (sFRP2) promotes osteosarcoma invasion and metastatic potential
Overexpression of sFRP2 in low metastatic human OS cells drives increased invasive potential. a CCK-8 cell proliferation assay of sFRP2/HOS and HOS vector control cells. Western blot analysis of whole cell lysates showing expression of sFRP2 in control and stable clones. b Cell migration assay of sFRP2/HOS and control HOS cells. c Transwell invasion assay of control HOS (top) and sFRP2/HOS (bottom) cells. Crystal violet staining for control and sFRP2 expressing cells is shown. Quantification of tumor cell invasion is shown to the right. d 3D matrigel assay for vector control (top panel) and sFRP2/HOS cells (bottom panel)