Fig. 2
From: Secreted Frizzled-Related Protein 2 (sFRP2) promotes osteosarcoma invasion and metastatic potential
Enhanced sFRP2 expression in non-metastatic mouse OS cells does not alter proliferation, but promotes increased migratory and invasive properties. a CCK-8 cell proliferation assay of sFRP2/RF43 and vector control cells. Western blot analysis of whole cell lysates showing expression of sFRP2 in control and sFRP2-overexpressing cells. Actin shown as loading control. b Cell migration assay of sFRP2/RF43 and control RF43 cells. c Transwell invasion assay of sFRP2/RF43 and control RF43 cells. Crystal violet staining for control and sFRP2 expressing cells is shown. Quantification of tumor cell invasion is shown to the right