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Fig. 4 | BMC Cancer

Fig. 4

From: Let-7 inhibits self-renewal of hepatocellular cancer stem-like cells through regulating the epithelial-mesenchymal transition and the Wnt signaling pathway

Fig. 4

The suppressions of EMT factors of HCC cells and Wnt activation of HCC stem cells were related to let-7a functions. a. Gene expression levels of let-7 targeted genes, which were implicated in cell apoptosis and cell cycle regulations. Let-7a decreased HMGA2, Bcl-xl, MAPK, cyclin d1, and increased Bcl-2 in both cell lines. b. Genes related to EMT were detected, and results showed that let-7 inhibited the EMT of HCC cells through regulating E-cadherin, N-cadherin and Snail, of which, E-cadherin was up-regulated, whereas, the mesenchymal biomarker N-cadherin and Snail were decreased. c. Let-7a1 inhibited the TCF-4 promoter activity of HCC cells, and inhibited Wnt1/Frizzled/β-catenin signaling of HCC stem cells, which was demonstrated to promote the self-renewal ability of cancer stem cells. d. The results of immunofluorescence showed that β-catenin was decreased due to let-7 overexpression in HCC stem cells

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BMC Cancer

ISSN: 1471-2407

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