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Fig. 3 | BMC Cancer

Fig. 3

From: Requirement of ABC transporter inhibition and Hoechst 33342 dye deprivation for the assessment of side population-defined C6 glioma stem cell metabolism using fluorescent probes

Fig. 3

MFI of JC-1 becomes comparable when ABC transporters are inhibited and Hoechst dye is deprived. a Removal of intracellular Hoechst 33342 dye by reculturing SP and MP cells. Twenty thousands of cells were sorted by FACS and recultured for 6 days. b FACS plots of SP- and MP-derived cells restainined with Hoechst 33342 dye. The mean percentages ± SD of SP-derived SP cells (left panel) or MP-derived SP cells (right panels) detected on day 6 of reculture to derive initial Hoechst 33342 were indicated in the plots. c FACS plots of SP- and MP-derived cells stained with JC-1 in the presence or absence of verapamil. d The mean fluorescent intensity of JC-1 aggregates was calculated and shown as bar graphs. n.s., not significant. n = 3

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