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Fig. 2 | BMC Cancer

Fig. 2

From: Requirement of ABC transporter inhibition and Hoechst 33342 dye deprivation for the assessment of side population-defined C6 glioma stem cell metabolism using fluorescent probes

Fig. 2

The influence of prestaining with Hoechst 33342 dye on the MFI of JC-1 aggregates. a FACS plots of C6 glioma cells stained with Hoechst 33342 dye and subsequently with JC-1 in the presence of verapamil. The upper and lower panels represent data from cells analyzed with or without Hoechst 33342-exciting 355 nm UV laser, respectively. The mean percentages ± SD of positive cells per total cells are shown in the plots. b Bar graph representing the mean fluorescent intensity of JC-1 aggregates of the JC-1-stained cells in (a). n = 3. c FSC/SSC plots of C6 glioma cells stained with (+) or without (−) Hoechst 33342 dye

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