Fig. 6
Treatment with Salinomycin interferes with Wnt/β-catenin signaling in human CD133+/CD133- colorectal cancer cells. The impact of Salinomycin treatment on the Wnt/β-catenin signaling pathway in SW620 CD133+/CD133- cells was investigated. For protein expression analysis 1 × 106 total SW620 (a), SW620-CD133+ (b) or SW620-CD133- (c) cells were cultured in 6-well plates in the absence or presence of 1 μM 5-FU or increasing concentrations of Salinomycin (1, 2, 5 and 10 μM). After protein extraction western blotting was performed using specific antibodies against total LRP6 and phosphorylated (P-LRP6). Results are displayed as western blot analysis of LRP6 and P-LRP6 (up) with densitometry quantitative analysis (down). * p < 0.05, ** p < 0.001 compared to control