Fig. 5

Salinomycin reduces proliferation and induces cell death of human CD133 colorectal cancer cells after treatment with Salinomycin. 5 × 10³ SW620 or SW620-CD133⁺/CD133^- (a) and HT29 or HT29-CD133⁺/CD133^- (b) cells were cultured in microtitre plates in the absence or presence of 1 μM 5-FU or increasing concentrations of Salinomycin (1, 2, 5 and 10 μM). Treatment was performed for 48 h. Tumor cell proliferation was investigated using the WST-1 assay. Results are displayed as summary or at least 3 independent experiments as mean ± SD; * p < 0.05 and ** p < 0.001 compared with control. For cell death detection 0.5 × 10⁶ SW620 or SW620-CD133⁺/CD133^- (c) and HT29 or HT29-CD133⁺/CD133^- (d) cells were cultured in 6-well plates in the absence or presence of 1 μM 5-FU or increasing concentrations of Salinomycin (1, 2, 5 and 10 μM). AnnexinV/PI staining was performed and cells were analyzed by flowcytometry. Results are displayed as summary or at least 3 independent experiments as mean ± SD; *p < 0.05 compared with control