Fig. 3
Induction of cell death of murine colorectal cancer cells after treatment with Salinomycin. 0.5 × 106 MC38 (a) or CT26 (b) cells were seeded in 6-well plates and grown until confluence following exposure to 1 μM 5-FU and increasing concentrations of Salinomycin (1 μM, 2 μM, 5 μM and 10 μM) for 24 h. Detection of cell death was performed using AnnexinV-FITC and PI staining and cells analyzed by flowcytometry. Cell death was further determined by quantification of DNA fragmentation (c + d), c-PARP production (e + f) and LDH release assay (g + h). Results are displayed as representative dot blots or as summary of at least 3 independent experiments; * p < 0.05 compared with control