Fig. 8

Expression and prognostic association of P4HA gene mRNAs. The expression of P4HA1 and P4HA2 was calculated in the stroma and cancer cells using the xenograft data (a) and in breast cancer and normal breast tissue normalized to the ECM metagene using the TCGA data (b). The P4HA3 data, also shown in Figs. 6 and 7 are included for comparison. Five breast cancer tumors were laser-microdissected and the mRNA levels of indicated genes were analyzed in the cancer cell and stroma compartments (c). The correlation coefficients of P4HA1, P4HA2, and P4HA3 with the ECM metagene in six different TCGA cancer sets were calculated (d). The hazard ratios of the standardized log2 mRNA expression of the P4HA genes were estimated using Cox proportional hazards model (e). The lines indicate 95 % confidence intervals of the hazard ratios in univariate models (dark colors) or multivariate models (bright colors) in which the standardized ECM metagene was included as variable. All models were stratified for node and ER status